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The Rural Voice, 1981-05, Page 29GUEST COLUMN Test your soil's atrazine level Robert Forrest. Agronomy Division. Cen- tralia College of Agricultural Technology. Any farmer who grows corn probably uses atrazine to control weeds in his crop. and if he practices crop rotation he must know the level of atrazine in his ;oil before rotating to a non -tolerant crop. Many farmers rent land from time to time. Very few rental agreements state the levels of pesticide residues in the soil. A simple bioassay would allow the rentor to determine if the residue was at a safe level for the crop he wished to grow. A simple bioassay may be conducted in ,a greenhouse or in the farm kitchen. A basic understanding of how atrazine works, however. is necessary in order to arrive at the proper conclusions. Atrazine is one of a family of triazine products and as such, it works within some plants to inhibit the photosynthetic process. Photosynthesis is the process within a plant that produces carbohydrate. and hence plant growth. If this process is disrupted, the plant ceases to grow and usually dies. Atrazine in the soil must be picked up by growing roots to gain entry into the plant. When seeds are planted in the soil the starch stored in the endosperm will support the growth of that seedling for some time. Barley, for example. is affected very little by atrazine until it runs out of starch in the seed, and its root system is established. Soil -applied atrazine breaks down through the activity of micro organisms. A small amount may leach to lower regions of the soil profile. The micro organisms that degrade or break down atrazine work most rapidly in warm. moist soil. Therefore the weather. length of growing season and time of application play an important role in the breakdown process. Since the micro organisms continue to work, although very slowly, through the fall, it is best to do a residue analysis in early spring. The procedure is not difficult. Use a soil probe and sample the top 15 cm of ;coi1(plow depth.) Collect about 7 or 8 litres of soil for each test. Place the soil in a plastic tray or pots with good drainage. Plant one third of each flat (or pots) with each of the three following crops: barley, white (or kidney) beans and cucumber. Plant a second flat (or pots) with the same three crops, but use soil of the same type, but known not to contain pesticide residues. Water lightly, as needed. Over -watering tends to move the atrazine residues out of the soil. Observe Inc seedlings daily for at least three weeks. Barley must reach the 5 -leaf stage. and beans must reach the 3-4 trifoliate leaf stage. This allows the root systems time to develop and take up any residues present. Atrazine damage causes a whitening of the tip of the oldest (lowest) leaf in barley. The whitening spreads along the edges of the leaf, and finally the leaf dies. Higher concentration of residue will cause damage that progresses upward on the plant. In beans and cucumber, similar whitening around the leaf margins results from atrazine uptake. This whitening then spreads over the remainder of the leaf. The plants grown in the residue -free soil should grow normally. They are included in the test to be sure that any symptoms noted on the plants grown in the suspect soil are indeed due to herbicide residues and not to other variables, such as over -watering, which would affect both. As a final check, add additional stress to the plants by reducing watering. Atrazine residues will affect plants more severely when they are under stress. The three different crop species are used to give an indication of the quantit y of any residue present. Barley is fairly tolerant to atrazine residue, while beans have considerably less tolerance, and cucumbers are highly susceptible to atrazine at very low levels. Using this simple test. a farmer can get a good indication of the amount of atrazine residue in his soil and can make intelligent decisions regarding the advisability of growing a sensitive crop on land previously treated with this herbicide. HOW IT WORKS; 20" disc. blades. mounted on individual pivots, are rotated by hydraulic motors to cut bean plants off below ground surface. Each pivot -mounted section rests on two depth wheels. Two or three windrows are produced, depending on the number of blades. The Smyth Bean Cutter can be mounted on the front or on the rear of a tractor. This compact machine is not bothered by mud or trash. Canada's first rotary bean cutter is a product of the George Smyth Welding and Machine Shop. THE FIRST NEW IDEA IN BEAN PULLING IN 70 YEARS SMYTN Welding and Machine Shop RR 2, Auburn, Ontario (519) 529-7212 THE RURAL VOICE/MAY 1981 PG 27